LC-MS-MS analytical parameters of 1-hydroxymidazolam and time-dependent inactivators
Analyte | 1-Hydroxymidazolam | Amprenavir | Diclofenac | Diltiazem | Erythromycin | Raloxifene | Troleandomycin |
|---|---|---|---|---|---|---|---|
| Mobile phase gradient program: %B (min) | 2(0) → 25 (0.6) → 55 | 2(0) → 40 | 2(0) → 25 | 2(0) → 25 | 2(0) → 30 | 2(0) → 2 | 2(0) → 25 |
| (3.3) → 55 (3.5) → 98 | (0.6) → 57 | (0.6) → 55 | (0.6) → 55 | (0.6) → 55 | (0.2) → 55 | (0.6) → 55 | |
| (4.2) → 2 (4.25) | (3.1) → 98 | (3.3) → 55 | (3.3) → 55 | (3.3) → 55 | (0.8) → 57 | (3.1) → 55 | |
| (4.2) → 98 | (3.5) → 98 | (3.5) → 98 | (3.4) → 98 | (3.1) → 98 | (3.5) → 98 | ||
| (5) → 2 (5.2) | (4.2) → 2 (4.25) | (4.2) → 2 (4.25) | (4.2) → 2 (4.25) | (4.2) → 98 | (4.2) → 2 (4.25) | ||
| (5) → 2 (5.2) | |||||||
| Analyte m/z transition | 342 → 324 | 506 → 245 | 296 → 214 | 415 → 370 | 716 → 558 | 474 → 112 | 772 → 586 |
| Mode | Positive | Positive | Positive | Positive | Positive | Positive | Positive |
| Capillary voltage (kV) | 2.50 | 2.52 | 2.52 | 2.52 | 2.52 | 2.52 | 2.52 |
| Cone voltage (V) | 40 | 32 | 50 | 36 | 35 | 30 | 17 |
| Desolvation temperature (°C) | 300 | 300 | 300 | 300 | 300 | 300 | 300 |
| Collision energy (eV) | 20 | 17 | 40 | 18 | 15 | 27 | 25 |
| Retention time (min) | 6.2 | 5.6 | 5.7 | 6.7 | 5.4 | 5.3 | 6.6 |