Primer sequence annealing temperatures for amplifying the promoter and coding regions of Cyp3A5 for sequencing
Exon | Primer Name | Primer Sequence | Annealing Temperature | Fragment Size |
|---|---|---|---|---|
| °C | base pairs | |||
| Promotera | Cyp3A5F | GAGGATGGATTTCAATTATTCTA | 55 | 387 |
| Cyp3A5R | GTCCATCGCCACTTTCCTTC | |||
| Exon 2 | 5Ex2f | CTCACCAGCCCTCTGATCTATAAA | 64 | 238 |
| 5Ex2r | AAGGAGGGGCATTTTTACTGATGG | |||
| Exon 3 | 5Ex3f | CCCCAATTAGAGGTAGGGTTTA | 60 | 175 |
| 5Ex3r | GGTAACCTCCTCACAGTAGC | |||
| Exon 4 | 5Ex4f | GGCTAGTGAAGTTTAATCAGCT | 56 | 190 |
| 5Ex4r | ATTTAATCAGTGGATCAATC | |||
| Exon 5 | 5Ex5f | TTGTGCAACCATGAAGATCA | 60 | 238 |
| 5Ex5r | CTTTCTACCTGTCCCCAGAT | |||
| Exon 6 | 5Ex6f | CCATATCCTTCTGGGACTTGAGTCTG | 64 | 215 |
| 5Ex6r | CATGGCAGGCAGCTGGAAGGG | |||
| Exon 7 | 5Ex7f | GCATGTATAGTGGAAGGACG | 60 | 275 |
| 5Ex7r | GAATTATATGTCAAGAAAGCAG | |||
| Exon 8 | 5Ex8f | GTAAAGTTTGCATTTTCAAT | 60 | 256 |
| 5Ex8r | CATGCTCTATCATGTGTATAA | |||
| Exon 9 | 5Ex9f | GCCATAGGGAGGTTGAGGGAGGGAA | 64 | 234 |
| 5Ex9r | CCTCCCTCTTAGTGTCCCCGCCAG | |||
| Exon 10 | 5Ex10f | CTAAGCTGTGATGTTGTACGTTG | 64 | 348 |
| 5Ex10r | TCCTACATTATGTCAGTGAAGGAATC | |||
| Exon 11 | 5Ex11f | CTTCACGAATACTATGATCATTTACC | 60 | 422 |
| 5Ex11r | GGCAGAATATGCTTGAACCAGC | |||
| Exon 12 | 5Ex12f | GGGTTGTTCTTAAGTAAGAACCCCT | 64 | 374 |
| 5Ex12r | GATGGATCCAAGTAGGTTCTTTGGCC | |||
| Exon 13 | 5Ex13f | GATACATGGTGTTAAGAGTCGT | 60 | 422 |
|
| 5Ex13r | TCCTTTATACTACATAATGCACAAC |
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