TABLE 1

Aldehyde oxidase and xanthine oxidase/xanthine dehydrogenase activities in skin cytosols from various mammals

Each value represents the mean ± S.D. of four animals. Other details are described under Materials and Methods.

Species
Aldehyde Oxidasea
Xanthine Oxidoreductase
Xanthine Oxidaseb
 Xanthine Dehydrogenasec
nmol/min/mg protein
Hamster 1.43 ± 0.26 0.15 ± 0.01 0.66 ± 0.14
Rabbit 0.91 ± 0.07 0.05 ± 0.01 0.24 ± 0.16
Guinea pig 0.47 ± 0.17 0.36 ± 0.12 1.81 ± 0.21
Mouse 0.26 ± 0.08 0.83 ± 0.14 1.95 ± 0.78
Rat
0.24 ± 0.11
1.23 ± 0.15
1.97 ± 0.39

  • ↵ a The mixture containing 0.1 μmol of benzaldehyde and skin cytosol in 0.1 M postassium/sodium phosphate buffer (pH 7.4) was incubated at 37°C for 15 min. Benzoic acid formed was determined using HPLC.

  • ↵ b The mixture containing 0.1 μmol of 1-methylxanthine and skin cytosol in 0.1 M potassium/sodium phosphate buffer (pH 7.4) was incubated at 37°C for 20 min. 1-Methyluric acid formed was determined using HPLC.

  • ↵ c The mixture containing 0.1 μmol of 1-methylxanthine, 0.27 μmol of NAD, and skin cytosol in 0.1 M potassium/sodium phosphate buffer (pH 7.4) was incubated at 37°C for 20 min. 1-Methyluric acid formed was determined using HPLC.