TABLE 1

Conditions for HPLC analysis

Compound
Column
Mobile Phase
Detection
Betamethasone Lichrospher RP-18a Acetonitrile/H2O (45:55) 1 ml/min UV 254 nm
Flurbiprofen Chiralpak ADb Hexane/2-propranol/TFA (95:5:0.5) 0.5 ml/min Fluorescence ex 247 nm, em 314 nm
Propranolol Chiralcel ODb Hexane/2-propranol (70:30) 1 ml/min Fluorescence ex 285 nm, em 340 nm
p-Aminobenzoic acid TSKgel ODS-80TMc Acetonitrile/H2O (20:80) 1 ml/min Fluorescence ex 291 nm, em 330 nm
o-Aminobenzoic acid TSKgel ODS-80TMc Acetonitrile/H2O (30:70) 1 ml/min Fluorescence ex 251 nm, em 394 nm
p-Hydroxybenzoic acid TSKgel ODS-80TMc Acetonitrile/H2O (20:80) 1 ml/min UV 252 nm
p-Nitrobenzoic acid
TSKgel ODS-80TMc
Acetonitrile/H2O (20:80) 1 ml/min
UV 274 nm

  • ↵ a 4.6 mm ϕ × 150 mm (Merck KGaA, Darmstadt, Germany).

  • ↵ b 4.6 mm ϕ × 250 mm (Daicel Chemical Industries, Ltd., Tokyo, Japan).

  • ↵ c 4.6 mm ϕ × 150 mm (TOSOH Co., Tokyo, Japan).