Hydrolysis of p-amino benzoic acid (BA) propyl ester by hCE-1 and hCE-2 in the presence and absence of alcohol
Protein concentrations were: hCE-1, 45 μg/ml; hCE-2, 25 μg/ml. Substrate concentration was 500 μM; EtOH and BuOH concentrations were 0.5 to 25 mM. Reaction time was 60 min and temperature, 37°C. Values represent the mean ± S.D.
hCE-1 | hCE-2 | ||||||
|---|---|---|---|---|---|---|---|
| Alcohol | p-Amino BA | p-Amino BA ester | p-Amino BA | p-Amino BA ester | |||
| mM | μM | μM | |||||
| Control | — | 6.29 ± 0.31 | 4.41 ± 0.27 | ||||
| 0.5 | 5.90 ± 0.80 | N.D. | 4.14 ± 0.13 | N.D. | |||
| EtOH | 5 | 4.78 ± 0.44 | 1.03 ± 0.06 | 4.10 ± 0.37 | 1.54 ± 0.08 | ||
| 10 | 4.26 ± 0.77 | 1.59 ± 0.55 | 3.97 ± 0.11 | 2.26 ± 0.30 | |||
| 25 | 3.05 ± 0.23 | 2.99 ± 0.55 | 3.68 ± 0.13 | 2.78 ± 0.13 | |||
| 0.5 | 5.76 ± 0.47 | N.D. | 3.72 ± 0.07 | N.D. | |||
| BuOH | 5 | 4.93 ± 0.46 | 1.24 ± 0.81 | 3.49 ± 0.10 | N.D. | ||
| 10 | 2.83 ± 0.88 | 3.30 ± 0.27 | 2.99 ± 0.31 | 0.33 ± 0.19 | |||
|
| 25 | 1.99 ± 0.37 | 4.97 ± 0.31 | 2.22 ± 0.84 | 0.83 ± 0.11 | ||
BA, benzoic acid; EtOH, ethanol; BuOH, butanol; N.D., not detected.