TABLE 1

Intraday (boldface) and interday (italicized) assay variability from five rifampicin concentration-response experiments conducted in triplicate


Experiment No.

1 μM RIF

10 μM RIF

30 μM RIF

50 μM RIF

Curve-Fit Parametersa
Average
CVb
Average
CV
Average
CV
Average
CV
EC50
Emax
r2
γ
% % % %
1 4.1 6 7.4 3 8.2 2 9.0 11 2.1 10 0.98 0.56
2 5.5 27 12 23 13 23 14 22 1.7 14 0.99 0.93
3 5.4 17 10 14 10 14 12 13 1.3 12 0.98 0.76
4 6.3 23 12 15 16 15 16 16 3.1 19 0.99 0.62
5 3.8 15 8.1 25 11 16 8.1 29 1.5 9.7 0.95 1.1
Average 5.0 9.9 12 12 1.9 13
CV
21%

22%

25%

28%

37%
28%


  • a Data were fit using a Hill function (three-parameter) according to the following equation: y = (Emax · xγ)/(EC50γ + xγ).

  • b CV = coefficient of variation (standard deviation/average).