Primer sequences for RT-qPCR assays
Gene | Forward Primer (5′- 3′) | Reverse Primer (5′- 3′) |
|---|---|---|
| Drug-metabolizing enzymes | ||
| CYP2B6 | TTCCTACTGCTTCCGTCTATCAAA | GTGCAGAATCCCACAGCTCA |
| CYP1B1 | GCTGCAGTGGCTGCTCCT | CCCACGACCTGATCCAATTCT |
| CYP3A4 | CTTCATCCAATGGACTGCATAAAT | TCCCAAGTATAACACTCTACACAGACAA |
| NQO1 | GCCGCAGACCTTGTGATATT | TGAACACTCGCTCAAACCAG |
| ABC transporters | ||
| MDR1/ABCB1 | GCCAAAGCCAAAATATCAGC | TTCCAATGTGTTCGGCATTA |
| MRP2/ABCC2 | TGAGCAAGTTTGAAACGCACAT | AGCTCTTCTCCTGCCGTCTCT |
| MRP3/ABCC3 | GTCCGCAGAATGGACTTGAT | TCACCACTTGGGGATCATTT |
| MRP6/ABCC6 | TGTCGCTCTTTGGAAAATCC | AGGAACACTGCGAAGCTCAT |
| BCRP/ABCG2 | TGCAACATGTACTGGCGAAGA | TCTTCCACAAGCCCCAGG |
| BSEP/ABCB11 | TGATCCTGATCAAGGGAAGG | TGGTTCCTGGGAAACAATTC |
| SLC transporters | ||
| OATP-A/SLCO1A2 | TGGGGAACTTTGAAATGTGG | AAGGCTGGAACAAAGCTTGA |
| OATP-B/SLCO2B1 | TGATTGGCTATGGGGCTATC | CATATCCTCAGGGCTGGTGT |
| OATP-C/SLCO1B1 | GCCCAAGAGATGATGCTTGT | ATTGAGTGGAAACCCAGTGC |
| OATP8/SLCO1B3 | GGGTGAATGCCCAAGAGATA | ATTGACTGGAAACCCATTGC |
| NTCP/SLC10A1 | GGGACATGAACCTCAGCATT | CGTTTGGATTTGAGGACGAT |
| OAT2/SLC22A7 | GAGGATGAACCTGCCACAGT | TCTGCTCACACACCAGATCC |
| OCT1/SLC22A1 | TAATGGACCACATCGCTCAA | AGCCCCTGATAGAGCACAGA |
| 18S | CGCCGCTAGAGGTGAAATTC | TTGGCAAATGCTTTCGCTC |