Effect of phenobarbital treatment on body weight, liver weight, liver lipids, serum lipids, serum glucose, and microsomal enzyme activity in SD-, SD+I-, HF-, and HF+I-fed rats for 8 weeks and then administered PB
Values are means ± S.E. of six to seven determinants.
Treatment | SD | SD+I | HF | HF+I | |
|---|---|---|---|---|---|
| Body weight (g) | C | 289.7 ± 5.3 | 279.2 ± 8.3 | 287.1 ± 4.4 | 258.5 ± 4.9**, # |
| PB | 285.9 ± 7.0 | 272.9 ± 6.6 | 281.6 ± 7.2 | 256.5 ± 7.9**, # | |
| Liver weight (g) | C | 9.80 ± 0.22 | 9.27 ± 0.23 | 14.07 ± 0.33*** | 12.51 ± 0.39***, ## |
| PB | 11.63 ± 0.15+++ | 11.55 ± 0.42+++ | 16.53 ± 0.63***, +++ | 15.14 ± 0.42***, +++ | |
| Ratio | |||||
| Liver weight/body weight | C | 0.032 ± 0.001 | 0.034 ± 0.001 | 0.051 ± 0.002 | 0.047 ± 0.002 |
| PB | 0.040 ± 0.001+++ | 0.042 ± 0.001++ | 0.059 ± 0.001+++ | 0.059 ± 0.003+++ | |
| Liver triacylglycerol (mg/g liver) | C | 12.93 ± 0.82 | 14.27 ± 0.34 | 72.55 ± 8.67*** | 44.80 ± 5.18***, # |
| PB | 21.25 ± 2.62 | 13.89 ± 0.43 | 103.57 ± 6.48***, +++ | 94.29 ± 6.63***, +++ | |
| Liver total cholesterol (mg/g liver) | C | 4.73 ± 0.92 | 3.29 ± 0.49 | 32.47 ± 3.74*** | 27.88 ± 5.52*** |
| PB | 5.95 ± 0.82 | 4.09 ± 0.70 | 26.78 ± 3.12*** | 32.21 ± 7.06*** | |
| Liver free fatty acid (μEq/g liver) | C | 8.97 ± 0.58 | 8.89 ± 0.69 | 49.86 ± 2.55*** | 43.76 ± 2.46***, # |
| PB | 11.08 ± 1.02 | 9.22 ± 0.48 | 36.50 ± 3.62***, +++ | 41.33 ± 4.83***, +++ | |
| Serum triacylglycerol (g/l) | C | 1.54 ± 0.12 | 1.47 ± 0.17 | 1.73 ± 0.25 | 1.11 ± 0.10# |
| PB | 0.94 ± 0.07 | 0.91 ± 0.05 | 2.10 ± 0.28*** | 1.83 ± 0.25** | |
| Serum total cholesterol (g/l) | C | 0.60 ± 0.02 | 0.57 ± 0.02 | 2.17 ± 0.29*** | 2.21 ± 0.17*** |
| PB | 0.90 ± 0.02 | 0.82 ± 0.04 | 1.78 ± 0.01*** | 1.92 ± 0.20*** | |
| Serum free fatty acid (μEq/l) | C | 462.6 ± 19.3 | 454.1 ± 33.4 | 831.1 ± 93.9*** | 651.9 ± 84.1*, # |
| PB | 451.3 ± 36.3 | 462.5 ± 40.7 | 905.5 ± 67.8*** | 827.6 ± 81.1*** | |
| Serum glucose (g/l) | C | 1.34 ± 0.07 | 1.34 ± 0.09 | 1.60 ± 0.11* | 1.35 ± 0.05# |
| PB | 1.21 ± 0.08 | 1.26 ± 0.04 | 1.41 ± 0.11 | 1.25 ± 0.07 | |
| Microsome protein (mg/g liver) | C | 25.4 ± 1.3 | 22.5 ± 1.6 | 26.9 ± 1.1 | 26.0 ± 1.0 |
| PB | 24.7 ± 1.5 | 23.7 ± 0.8 | 25.9 ± 2.7 | 27.8 ± 0.7 | |
| Pentoxyresorufin O-dealkylation (pmol/mg microsome protein/min) | C | 10.0 ± 1.9 | 12.7 ± 2.3 | 8.0 ± 1.2 | 7.1 ± 0.6 |
| PB | 813.5 ± 51.5+++ | 786.8 ± 81.6+++ | 751.5 ± 61.3+++ | 640.6 ± 49.8*, +++ |
C, vehicle.
↵* p < 0.05, ** p < 0.01, *** p < 0.001 for SD + I-, HF-, or HF + I-fed and vehicle-administered animals versus SD-fed and vehicle-administered animals, or SD + I-, HF-, or HF + I-fed and PB-administered animals versus SD-fed and PB-administered animals.
↵++ p < 0.01, +++p < 0.001 for PB-administered animals versus vehicle-administered animals.
↵# p < 0.05, ##p < 0.01 for HF + I-fed and vehicle-administered animals versus HF-fed and vehicle-administered animals or HF + I-fed and PB-administered animals versus HF-fed and PB-administered animals.