Inhibitory effects of propofol on testosterone 16α-, 16β-, and 6β-hydroxylation (CYP2C, CYP2B, and CYP3A, respectively) in liver microsomes from untreated male rats
Testosterone (50 μM) was incubated at 37°C for 10 min with male rat liver microsomes in the absence or presence of propofol (20 μM). Data are means ± S.D. from three untreated male rats.
Hydroxylation | Testosterone Hydroxylation (%) | ||
|---|---|---|---|
| Without Propofol | With Propofol | ||
| nmol/min/mg protein | |||
| 16α | 0.99 ± 0.12 (100) | 0.47 ± 0.07 (47)* | |
| 16β | 0.31 ± 0.03 (100) | 0.08 ± 0.02 (26)* | |
| 6β | 1.82 ± 0.18 (100) | 1.55 ± 0.22 (85) | |
↵* p < 0.01, significant difference from the control (without propofol).