Sequences of primers used for semiquantitative RT-PCR
Gene (NCBI GenBank) | Forward Primer | Reverse Primer | Size/Annealing Temperature |
|---|---|---|---|
| bp/°C | |||
| Acyl CoA oxidase (AF185048) | CTCGCAGACCCAGATGAAAT | TCCAAGCCTCGAAGATGAGT | 218/60 |
| Albumin (X12422) | GCACGAGAAGACACCAGTGA | CGAGTGCAGTTTGCTTCTTG | 200/62 |
| CPT-I (AF288789) | GCATTTGTCCCATCTTTCGT | GCACTGGTCCTTCTGGGATA | 198/60 |
| Cathepsin B (AJ315560) | GGCCTCTATGACTCGCATGT | GCAAGTTCCCCTCAAGTCTG | 198/60 |
| Dual oxidase 2 (AF547267) | GACCCAGCGGCAGTTTGAATGG | AGGGCCGCAGCTGAACACTCC | 295/64 |
| GAPDH (AF017079) | AGGGGCTCTCCAGAACATCATCC | TCGCGTGCTCTTGCTGGGGTTGG | 446/60 |
| Sodium iodide symporter (AJ487855) | AGTCATCAGCGGCCCCCTCCTC | ACCGATGCCGTCTGCCGTGTG | 456/60 |
| Thyroglobulin (AF165610) | CAGTAAGGGCTTCCGTCTTG | GGAGCTGCACTGAGGAATGT | 198/60 |
| Thyroid hormone receptor α1 (AJ005797) | CCAGATGGAAAGCGAAAAAG | TGGGATGGAGATTCTTCTGG | 199/60 |
| Thyroid peroxidase (X04645) | CTGGGCGCCGTGCTCGTCTG | ACGCGGGTGGCATCTGACTCTGAC | 287/65 |
| Thyroxine-binding globulin (NM214058) | GTGGCTTCTTGGGCATGTAT | GAACCTCCGGTACAGGTTGA | 206/62 |
| Transthyretin (X87846) | ATGGTCAAAGTCCTGGATGC | TGCCTTCCAGTAGGATTTGG | 207/60 |
| TSH receptor (NM214297) | GCCTGCCCATGGACACTGAGAC | CTGACCCCGGTATGCCTGAGC | 422/60 |
| Type I iodothyronine deiodinase (AY533206) | CTCTGGGTGCTCTTTCAGGT | ATCGGACCTTCAGCACAAAC | 199/62 |
| Type II iodothyronine deiodinase (NM001001626) | CTCGGTCATTCTCCTCAAGC | TGCTTCCTTCAGGATTGGAG | 200/60 |