Recombinant Human P450 Enzymes | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HLM | 1A1 | 1A2 | 4F2 | 2J2 | 1B1 | 4F3B | 4F12 | 2C8 | |||||||||
Apparent Km (μM) | 0.5 | 0.5 | 0.4 | 0.7 | 0.9 | 0.3 | 3.2 | 1.6 | 2.6 | ||||||||
Vmax (nmol/min/nmol P450) | 10.5a (3.8) | 26 | 13 | 7.9 | 7.7 | 2.2 | 10 | 0.19 | 0.12 | ||||||||
CLint (μl/min/pmol P450) | 21 | 52 | 32 | 11 | 8.6 | 6.3 | 3.1 | 0.12 | 0.046 | ||||||||
Incubation time (min) | 3 | 2 | 2 | 5 | 5 | 5 | 15 | 15 | 15 | ||||||||
Protein concentration (pmol P450/ml) | 7.2a (0.02) | 10 | 10 | 10 | 10 | 10 | 10 | 25 | 50 | ||||||||
DB289 concentration range (μM) | 0.05-15 | 0.05-25 | 0.05-5 | 0.05-5 | 0.05-5 | 0.05-25 | 0.05-25 | 0.05-25 | 0.05-25 |
↵ a For HLMs, the P450 enzyme concentration is the total P450 enzyme concentration. The unit for the value in the parentheses is nmol/min/mg protein for Vmax and mg/ml for protein concentration, respectively.