Recombinant Human P450 Enzymes | |||||||
---|---|---|---|---|---|---|---|
3A4 | 2D6 | 2E1 | 3A5 | ||||
S50 (μM) | 3.7 | 2.8 | 2.9 | 4.7 | |||
Vmax (nmol/min/nmol P450) | 0.72 | 0.35 | 0.03 | 0.06 | |||
n (Hill coefficient) | 2.2 | 1.9 | 1.4 | 2.7 | |||
CLmax (μl/min/pmol P450) | 0.098 | 0.063 | 0.007 | 0.006 | |||
Incubation time (min) | 15 | 15 | 15 | 15 | |||
Protein concentration (pmol P450/ml) | 50 | 20 | 50 | 50 |