Steady-state kinetics: H2O2- and NADPH-supported oxidation of 7-BQ by CYP3A4 and random mutants
Results are the mean ± standard deviation of at least three independent experiments.
CYP3A4 | kcat | S50, 7-BQ | n |
|---|---|---|---|
| min-1 | μM | ||
| H2O2-supported | |||
| WT | 1.6 ± 0.06 | 105 ± 7.0 | 2.1 ± 0.10 |
| L216W | 2.6 ± 0.17 | 139 ± 5.2 | 1.8 ± 0.06 |
| F241V | 1.9 ± 0.04 | 107 ± 3.4 | 2.3 ± 0.14 |
| F228I | 2.9 ± 0.60 | 161 ± 52 | 1.2 ± 0.13 |
| T433S | 2.7 ± 0.80 | 95.2 ± 14 | 1.7 ± 0.12 |
| NADPH-supported | |||
| WT | 43 ± 14 | 228 ± 60 | 2.8 ± 0.32 |
| L216W | 37 ± 10 | 276 ± 60 | 2.2 ± 0.31 |
| F241V | 25 ± 6.0 | 158 ± 30 | 3.1 ± 0.33 |
| F228I | 29 ± 8.9 | 316 ± 14 | 1.0 ± 0.10 |
| T433S | 39 ± 10 | 132 ± 11 | 1.5 ± 0.14 |