TABLE 2

Mean covalent binding of [3H]raloxifene and [14C]naphthalene in freshly isolated hepatocytes

All experiments were performed in triplicate, and the statistics were done using the t test function in Excel.

Fresh Lot 1
Fresh Lot 2
Fresh Lot 3
Average
pmol/mg protein
[3H]Raloxifene
    Control (t = 120 min) 74.3 ± 7.3 109 ± 17 63.1 ± 3.7 82.3 ± 16
    100 μM Verapamil 106 ± 8* 165 ± 11* 111 ± 15* 127 ± 22**
    1 μM Ketoconazole
29.2 ± 3.3**
39.3 ± 4.9**
24.4 ± 1.6**
31.0 ± 6.5**
Fresh Lot 4
Fresh Lot 5
Fresh Lot 6
Average
[14C]Naphthalene
    Control (t = 120 min) 224 ± 28 165 ± 21 240 ± 38 210 ± 50
    100 μM Verapamil 261 ± 6 223 ± 20 176 ± 18 220 ± 30
    1 μM Ketoconazole 234 ± 2 188 ± 15 163 ± 10 195 ± 20
    500 μM 4-Methylpyrazone
149 ± 4*
96.6 ± 23.1*
109 ± 7**
118 ± 30**

  • ↵* p < 0.01; ** p < 0.005.