TABLE 3

Effect of flavonol glycosides and aglycones on CYP2B1 and CYP3A23 mRNA expression in primary cultures of rat hepatocytes

Primary cultures of rat hepatocytes were treated with a G. biloba extract (i.e., extract A, Table 1; 100 μg/ml), kaempferol-3-O-rutinoside (1.9 μg/ml), quercetin-3-O-rutinoside (4 μg/ml), isorhamnetin-3-O-rutinoside (0.6 μg/ml), kaempferol (6.3 μg/ml), quercetin (10.6 μg/ml), or isorhamnetin (4.1 μg/ml). Control hepatocytes were treated with culture medium (vehicle for the extract) or DMSO (0.1%, vehicle for the individual chemicals). Data are expressed as the mean ± S.E.M. fold-increase in mRNA expression (relative to the vehicle-treated control group) for hepatocyte cultures from three individual rats.