TABLE 2

Validation of CyA measurements in tissue homogenates

CyA was added to tissue homogenates to achieve a final concentration of 10, 100, or 1000 ng/ml. Samples were then extracted and CyA was measured by HPLC-MS/MS to validate measurement of that drug in the tissue homogenates.

Brain			Liver			Kidney
[CyA] Added	[CyA] Detected	[CyA] Added	[CyA] Detected	[CyA] Added	[CyA] Detected
ng/ml			ng/ml			ng/ml
10	10.4 ± 0.76^a	100	96 (n = 2)^b	100	100.2 (n = 2)^b
100	98.5 ± 3.12^a	1000	994 ± 80^a	1000	1056 ± 31^a

↵ a These values are expressed as the mean (n = 3) ± S.E.M.
↵ b These values represent the mean value of two samples.