Recovery percentage of PL and isovaleryl-PL at steady state in rat jejunal single-pass perfusion
The mucosal concentration of PL was 376 ± 15.0 nmol/g tissue under PL perfusion, and those of PL and isovaleryl-PL were 436 ± 89.2 and 97.7 ± 11.0 nmol/g tissue, respectively, under isovaleryl-PL perfusion. PL or isovaleryl-PL (300 μM) was perfused in the jejunal lumen at 0.3 ml/min. The flow rate of vascular perfusate was 2.8 ml/min. The samples of luminal and vascular perfusate were collected at 10- and 5-min intervals, respectively, for 60 min. Values represent mean ± S.D. (n = 3).
Perfusate | Recovery Percentage of Perfused Amount | ||
|---|---|---|---|
| PL | Isovaleryl-PL | ||
| PL | |||
| Mesenteric blood | 6.81 ± 0.21 | ||
| Intestinal fluid | 92.1 ± 0.71 | ||
| Totala | 98.9 ± 0.91 | ||
| Mucosal tissueb | 4.76 ± 0.01 | ||
| Isovaleryl-PL | |||
| Mesenteric blood | 7.47 ± 1.33 | 0.51 ± 0.17 | |
| Intestinal fluid | 44.6 ± 3.29 | 44.6 ± 7.48 | |
| Totala | 97.4 ± 5.03 | ||
| Mucosal tissueb | 7.14 ± 2.01 | 1.58 ± 0.20 | |