TABLE 5

Concentrations in plasma and tissue of NMRI mice treated with either 0.80 mmol/kg S-2-pentyl-4-pentynoic hydroxamic acid (i.p.) or 1.50 mmol/kg S-2-pentyl-4-pentynoic acid (s.c.)

Concentrations ± S.D. at Time Points (min) after Dosage (μg/ml or μg/g)
0
7
15
30
60
120
240
360
720
S-2-Pentyl-4-pentynoic hydroxamic acid
    Plasma <LOD 124 ± 12 157 ± 44 58 ± 26 41 ± 10 <LOD <LOD <LOD <LOD
    Brain <LOD 141 ± 12 186 ± 42 74 ± 8 38 ± 14 <LOD <LOD <LOD <LOD
    Liver <LOD 72 ± 6 67 ± 35 23 ± 16 <LOQ <LOD <LOD <LOD <LOD
    Kidney N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D.
    Spleen <LOD 106 ± 11 120 ± 67 <LOQ 25a <LOD <LOD <LOD <LOD
The metabolite S-2-pentyl-4-pentynoic acid
    Plasma <LOD 41 ± 2 65 ± 6 112 ± 10 106 ± 21 114 ± 5 29 ± 8 32 ± 14 <LOD
    Brain <LOD 14 ± 3 18 ± 8 29 ± 11 24 ± 7 20 ± 2 <LOQ <LOQ <LOD
    Liver <LOD 156 ± 2 122 ± 28 123 ± 18 95 ± 3 58 ± 5 28 ± 8 34 ± 15 <LOQ
    Kidney <LOD 65 ± 7 61 ± 13 78 ± 14 75 ± 14 74 ± 8 14 ± 4 44 ± 31 <LOD
    Spleen <LOD 16 ± 8 24 ± 13 42 ± 18 42 ± 28 65 ± 14 <LOQ <LOQ <LOD
S-2-Pentyl-4-pentynoic acid
    Plasma <LOD 501 ± 85 530 ± 64 570 ± 55 555 ± 30 439 ± 8 226 ± 70 267 ± 18 <LOQ
    Brain <LOD 217 ± 101 191 ± 38 233 ± 8 242 ± 14 171 ± 12 52 ± 29 47 ± 14 <LOQ
    Liver <LOD 375 ± 57 368 ± 79 421 ± 80 617 ± 66 411 ± 56 201 ± 50 143 ± 32 38 ± 21
    Kidney <LOD 223 ± 34 300 ± 88 275 ± 11 514 ± 110 352 ± 84 162 ± 80 165 ± 48 17a
    Spleen
<LOD
150 ± 16
154 ± 43
225 ± 48
416 ± 75
221 ± 41
83 ± 52
68 ± 17
<LOQ