Primer sequences for quantitative RT-PCR
Name | Primers | 5′-3′ Sequence | Amplicon | Accession No. |
|---|---|---|---|---|
| base pairs | ||||
| Cyp2a5 | Forward | ATGCTGACCTCAGGACTCCTC | 217 | NP_031838 |
| Reverse | GGTAGATGGTGAATACAGGACCA | |||
| Cyp2b10 | Forward | ACCCCACGTTCCTCTTCCA | 100 | NM_009999 |
| Reverse | CAGCAGGCGCAAGAACTGA | |||
| Cyp2c29 | Forward | GCTCAAAGCCTACTGTCA | 179 | NM_007815 |
| Reverse | CATGAGTGTAAATCGTCTCA | |||
| Cyp2d9 | Forward | TGGCACAGATAGAGAAGGCCA | 76 | NM_010006 |
| Reverse | TCACGCACCACCATGAGC | |||
| Cyp3a11 | Forward | GGATGAGATCGATGAGGCTCTG | 73 | NM_007818 |
| Reverse | CAGGTATTCCATCTCCATCACAGT | |||
| Cyp7a1 | Forward | AGCATTTCTTTGATCTGGGGG | 185 | NP_031850 |
| Reverse | CCATGTTTCCTTTGCTTTGCTCT | |||
| HPRT | Forward | GCTTTCCCTGGTTAAGCAGTACA | 125 | MUSHPRT |
| Reverse | CAAACTTGTCTGGAATTTCAAATC | |||
| Albumin | Forward | GACTTTGCACAGTTCCTGGATACA | 125 | MMU457860 |
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| Reverse | TTGTGGTTGTGATGTGTTTAGGCTA |
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