3MI and metabolite standards used to identify peaks at specific retention times on HPLC
Standard | Detector | Concentration of Standard | Present in Cultured Cells | Typical Retention Time |
|---|---|---|---|---|
| μM | min | |||
| 3-Methyloxindole | UV | 15 | Yes | 15.7 |
| 3-Hydroxy-3-methyloxindole | UV | 45 | Yes | 9.8 |
| 2-Aminoacetophenone | UV | 45 | Yes | 18.8 |
| 5-Hydroxy-3-methylindole | FL | 15 | No | 13.7 |
| 6-Hydroxy-3-methylindole | FL | 15 | No | 16.6 |
| Indole-3-carbinol | FL | 15 | No | 13.0 |
| 6-Hydroxy-3-methylindole sulfate | FL | 15 | No | 15.9 |
| 3-Methylindole | FL | 15 | Yes | 25.5 |