Recovery of viable hepatocytes after cold preservation and cryopreservation/thawing
Hepatocyte viability was assessed by trypan blue exclusion before and after cold storage or cryopreservation. From this, the recovery of viable cells was calculated and expressed as a percentage of viable cells before preservation.
Donor No. 1 | Donor No. 2 | Donor No. 3 | |
|---|---|---|---|
| % | |||
| Recovery of viable cells after 3 h in UW solution | 55 | 97 | 66 |
| Recovery of viable cells after 24 h in UW solution | 35 | 56 | 61 |
| Recovery of viable cells after cryopreservation | 10 | 54 | 49 |