TABLE 4

Chromatographic retention times and mass spectral characteristics of TPA023 metabolites identified in urine, feces, and plasma of five healthy male subjects after a single oral 3-mg (99-μCi) dose of [14C]TPA023

Metabolites of TPA023
Description of Metabolites
Retention Time (tR)
m/z of [M + H]+ Ion
m/z of Major CID Fragments
min
M1 Glucuronidation of M14a 11 463 287
M2 Glucuronidation of M12 11 544 287, 368
M3 Glucuronidation of M11a 11.5 588 110, 285, 382, 412
M4 Glucuronidation of M9a 12.5 560 82, 285, 354, 384
M5 Glucuronidation of TPA023 14.5 572 110, 287, 396
M6 Glucuronidation of M12 16 544 287, 368
M7 Glucuronidation of M12 17.2 544 287, 368
M8 Glucuronidation of M11a 17.2 588 110, 285, 382, 412
M9 t-Butyl hydroxylation and N-desethylation of TPA023 18.5 412 82, 285, 354, 384
M10 Carboxylic acid of TPA023 21.5 426 110, 271, 299, 382
M11 t-Butyl hydroxylation of TPA023 23 412 110, 275, 287, 382
M12 N-desethylation of TPA023 27 368 82, 273, 287
M13 Hydroxylation of TPA023 27.7 412 110, 303
M14
O-Dealkylation of TPA023
34.5
287
228, 271

  • ↵ a Site of glucuronidation was not determined