TABLE 4

Relative amounts (arbitrary units of CYP3A mRNA/μg total RNA) of CYP3A mRNA in liver and intestine of male mouse The percentage of mRNA of the corresponding P450 isoforms detected in liver is given in parentheses. Total RNA (3.5 ng) was loaded for real-time RT-PCR assay, and the levels of P450 mRNA were corrected by β-actin mRNA expression to normalize RNA loading. Results are means of three animals ± S.E.M.



Cytochrome P450

CYP3A11
CYP3A13
CYP3A25
CYP3A41
Liver 5.4 ± 1.07 0.56 ± 0.12 5.28 ± 1.38 11.9 ± 2.9
Duodenum 0.32 ± 0.08 1.13 ± 0.33 0.55 ± 0.09 0.65 ± 0.18
(6%) (202%) (10%) (5%)
Ileum 0.12 ± 0.07 0.58 ± 0.29 0.19 ± 0.05 0.22 ± 0.14
(2%) (103%) (4%) (2%)
Colon 0.001 ± 0.0003 0.26 ± 0.08 0.0068 ± 0.004 0.004 ± 0.001

(0.02%)
(46%)
(0.12%)
(0.03%)