TABLE 1

P-gp transport kinetics of BCECF-AM in various BBB models

BBB Model
Km
Vmax
Vmax/Km
μM pmol · min–1 · mg protein–1 μl · min–1 · mg protein–1
BBM 2.68 ± 0.83 428.9 ± 85.6 202.4 ± 69.6
MDCKII-MDR1 1.58 ± 0.26 48.2 ± 20.6 32.6 ± 12.9
BBMEC
2.78 ± 0.36
100.2 ± 5.8
36.9 ± 6.8

  • Values represent mean ± S.E.M. (n = 9) from three separate studies