TABLE 2

MRP-related transport kinetics of BCECF in various BBB models

BBB Model
Km
Vmax
Vmax/Km
μM pmol · min–1 · mg protein–1 μl · min–1 · mg protein–1
BBM 1448.2 ± 183.1 624.9 ± 38.2 0.447 ± 0.083
MDCKII-MDR1 178.4 ± 12.4 92.5 ± 3.6 0.521 ± 0.023
BBMEC 474.3 ± 98.1 122.8 ± 22.9 0.262 ± 0.015
BBMEC + indomethacin
1299.6 ± 94.2
181.1 ± 4.5
0.141 ± 0.014

  • Values represent mean ± S.E.M. (n = 9) from three separate studies