TABLE 1

Correlation of formation rates of endosulfan sulfate from endosulfan isomers (10 μM) with the P450 marker activities in human liver microsomes (n = 14)

Data were analyzed using the nonparametric correlation test (Spearman r). The activity of each isoform was determined using the respective specific substrate probe reaction, as described previously (Kim et al., 2005b).

Activity
P450 Isoforms
Correlation Coefficient (r)
α-Endosulfan
β-Endosulfan
Phenacetin O-deethylation CYP1A2 0.55 0.54
Coumarin 7-hydroxylation CYP2A6 0.28 0.14
Bupropion hydroxylation CYP2B6 0.87* 0.46
Paclitaxel 6α-hydroxylation CYP2C8 0.65 0.36
Tolbutamide 4-methylhydroxylation CYP2C9 0.58 0.35
S-Mephenytoin N-demethylation CYP2C19 0.20 0.32
Dextromethorphan O-demethylation CYP2D6 0.10 0.14
Chlorzoxazone 6-hydroxylation CYP2E1 0.56 0.62
Midazolam 1′-hydroxylation CYP3A 0.68* 0.79*
Testosterone 6β-hydroxylation
CYP3A
0.73*
0.88*

  • ↵* p < 0.05.