Mean enzyme kinetic parameters of the formation of endosulfan sulfate from endosulfan isomers from the cDNA-expressed P450s
CYP2B6 | CYP3A4 | CYP3A5 | Total CLint | |
|---|---|---|---|---|
| α-Endosulfan | ||||
| Vmax (pmol/min/pmol P450 isoform) | 10.31 | 2.04 | 0.50 | |
| Km (μM) | 5.58 | 3.30 | 10.54 | |
| CLint [Vmax/Km (μl/min/pmol P450)] | 1.85 | 0.62 | 0.05 | 2.52 |
| n | 0.23 | 0.97 | 0.98 | |
| β-Endosulfan | ||||
| Vmax (pmol/min/pmol P450 isoform) | 34.75 | 25.36 | ||
| Km (μM) | 7.25 | 4.48 | ||
| CLint [Vmax/Km (μl/min/pmol P450)] | 4.80 | 5.66 | 10.46 | |
| n |
| 1.26 | 1.29 |
|