Primers used for amplification, sequencing, and directed mutagenesis
Primers | Sequences |
|---|---|
| R503 | 5′-ctaaattccagcaggctatc-3′ |
| F506 | 5′-gtaggtcttttacatttccc-3′ |
| R507 | 5′-cctgaaacagcaaaaccaa-3′ |
| R670-HindIII | 5′-ctagctgaagcttatcagagaactgcagctgaga-3′ |
| F838 | 5′-ttgcttccaagtctttg-3′ |
| R839 | 5′-attggggcactattcacaaaag-3′ |
| F840 | 5′-tttacagtcccacaaaaacaag-3′ |
| R841 | 5′-tcagatgagtagattccaaaatt-3′ |
| F842 | 5′-acctcagttttagtttgattacc-3′ |
| F1121-XhoI | 5′-ctagcagctcgagttgggtaaatcattgtcagtgac-3′ |
| F1154 | 5′-gagatcaaatttcaacacttg-3′ |
| F1155 | 5′-tcagtgactgattttttttttatgaaaggat-3′ |
| F1159 | 5′-gcagcatgttttacaatcctttg-3′ |
| R1164 | 5′-gtagattccaaaattttctc-3′ |
| R1165 | 5′-catgtacagactggtaaag-3′ |
| R1166 | 5′-atcctttcataaaaaaaaaatcagtcactga-3′ |
| F1293 | 5′-caccttaagaatacttcacctttgg-3′ |
| R1298 | 5′-caactaagataaaagcaagaatatgg-3′ |
| F1473-SmaI | 5′-ctagcagcccgggctcatatattcttgttcttttg-3′ |
| R1474-SmaI | 5′-ctagcagcccgggcagagaactgcagctgaga-3′ |
Restriction sites are underlined.