Serum level of DNP-SG, and GST activity and glutathione content in liver, jejunum, and renal cortex
Level of DNP-SG in serum was assessed by HPLC, 5 min after administration of CDNB. GST activity toward CDNB was assessed in cytosolic fractions. Tissue content of glutathione was assessed in homogenates from hepatic and extrahepatic tissues. Data are means ± S.D. Sham group consists of animals studied at day 1 and 7 (three each).
Sham (n = 6) | BDL1 (n = 3) | BDL7 (n = 3) | |
|---|---|---|---|
| Serum concentration of DNP-SG (mM) | 0.024 ± 0.004 | 0.023 ± 0.002 | 0.019 ± 0.004 |
| Liver GST activity (nmol/min/mg protein) | 1610 ± 81 | 1228 ± 100* | 1018 ± 236* |
| Intestinal GST activity (nmol/min/mg protein) | 443 ± 51 | 516 ± 37 | 512 ± 9 |
| Renal GST activity (nmol/min/mg protein) | 352 ± 10 | 430 ± 34* | 500 ± 12*,† |
| Hepatic total glutathione content (nmol/g liver) | 4544 ± 579 | 3193 ± 171* | 2764 ± 744* |
| Hepatic GSSG content (nmol/g liver) | 109 ± 30 | 238 ± 12* | 276 ± 59* |
| Intestinal total glutathione content (nmol/g liver) | 1958 ± 59 | 1115 ± 122* | 1274 ± 113* |
| Intestinal GSSG content (nmol/g liver) | 27 ± 4 | 36 ± 4* | 30 ± 2 |
| Renal total glutathione content (nmol/g liver) | 462 ± 35 | 614 ± 62 | 1174 ± 168*,† |
| Renal GSSG content (nmol/g liver) | 18 ± 1 | 38 ± 4* | 71 ± 10*,† |