TABLE 3

Carbon chemical shift assignments of vincristine, M2, and M3.

Position
δC
Vincristinea,b
 M2b
 M2c,d
 M3b
2 130.5 N.D. N.D. 134.1
3 117.5 119.0 N.D. 111.6
4 129.9 129.0 N.D. 128.8
5 118.6 118.2 116.9 118.8
6 119.8 119.8 118.0 119.7
7 123.1 122.9 120.8 122.7
8 112.2 112.3 110.9 111.4
9 136.9 137.2 N.D. 136.6
10 26.1 25.6 24.5 20.6
11 57.4 50.7 48.2 55.7
13 63.3 165.5 162.5
14 69.1 211.9 N.D. 212.5
15 39.8 50.1 47.9 46.2
16 29.6 30.4 29.0 27.9
17 47.0 52.6 N.D. 57.1
18 35.7 38.7 36.7 41.2
19 57.0 N.D. N.D. 57.7
20 35.5 35.8 33.8 36.7
21 6.9 7.8 6.8 7.7
22 176.2 175.5 N.D. 185.1
23 52.6 52.5 50.9
2′ 72.8 72.7 71.8 72.8
3′ 54.0 54.0 N.D. 53.7
4′ 125.2 124.9 N.D. 124.7
5′ 125.9 128.2 125.2 125.5
6′ 129.1 127.8 N.D. 129.4
7′ 159.4 159.1 N.D. 159.5
8′ 96.6 96.1 N.D. 96.9
9′ 142.5 142.5 N.D. 141.6
10′ 42.3 41.3 39.9 41.0
11′ 49.3 49.5 48.4 50.1
13′ 50.7 50.6 49.1 50.7
14′ 125.7 125.8 123.6 125.4
15′ 130.8 130.9 129.4 130.8
16′ 43.7 43.3 N.D. 43.3
17′ 64.8 64.0 63.2 65.1
18′ 77.9 78.4 76.1 77.8
19′ 80.7 80.9 N.D. 80.5
20′ 31.7 31.3 29.8 31.1
21′ 8.4 7.5 6.4 6.5
22′ 162.8 162.4 N.D. 162.8
23′ 56.6 56.4 55.5 56.1
24′ 171.6 171.9 N.D. 171.0
25′ 20.5 20.5 19.9 20.4
26′ 171.8 171.6 N.D. 171.7
27′
52.8
52.8
51.5
52.9

  • N.D., not detected.

  • ↵ a Vincristine used was isolated from the CYP3A5 incubation mixture.

  • ↵ b In CD3OD at 25°C.

  • ↵ c In DMSO-d6 at 100°C.

  • ↵ d Only carbons bearing protons were assigned.