TABLE 1

Percentage of enzyme and alkaline hydrolysis of PA-457Gs in rat bile

Acyl Glucuronides
β-Glucuronidasea
NaOHb
pH 4.0
pH 7.4
pH 9.0
mono-PA-457G (I) 100 99.7 99.8 84.0
mono-PA-457G (II) 100 99.2 94.6 90.3
di-PA-457G
100
100
100
100

  • ↵ a PA-457Gs from rat bile were preincubated in 0.1 M phosphate buffer (pH 4.0, 7.4, and 9.0) at 37°C for 72 h and then hydrolyzed with β-glucuronidase (4000 U/ml) at pH 5.0 and 37°C for 2 h.

  • ↵ b PA-457Gs from rat bile were hydrolyzed with 1 M NaOH at 60°C for 2 h.