ASA conditions for detection of each SNP in UGT1A3
SNPs | Allele-Specific Primer | Allele-Nonspecific Primer | Annealing and Extension Temperature | Length of Product |
|---|---|---|---|---|
| °C | bp | |||
| 17 | AGATGGCCACAGGACTCCAAGATGGCCACAGGACTCCG | AGCTCCACACAAGACCTATGATA | 68.5 | 391 |
| 31 | CAGTCCTGAGGCCAGCCACAGTCCTGAGGCCAGCCG | TGAGCACAGGGTCAGACGTG | 69.5 | 289 |
| 81 | CCACCAACACCTTTCCACTCCCACCAACACCTTTCCACTT | TGAGCACAGGGTCAGACGTG | 68.0 | 339 |
| 133 | CCCGCAAGACCTCCCGCCCGCAAGACCTCCCA | TGAGCACAGGGTCAGACGTG | 68.3 | 388 |
| 140 | CTCAGCATGCGGGAGGCCTCAGCATGCGGGAGGT | AGCTCCACACAAGACCTATGATA | 69.5 | 268 |
| 470 | CGTTAACCTCTGCGCGGCACGTTAACCTCTGCGCGGCG | GAAGACCATGTTGGGCATGATT | 72.8 | 387 |
bp, base pairs.