TABLE 1

Precision and accuracy of the HPLC method for the analysis of propranolol glucuronides

The accuracy was calculated as the ratio of measured concentration and nominal concentration × 100%.

Nominal conc.
S-Propranolol Glucuronide
R-Propranolol Glucuronide
Measured conc.
Precision RSD (n = 5)
Accuracy (n = 5)
Measured conc.
Precision RSD (n = 5)
Accuracy (n = 5)
nM % nM %
5 nM 5.3 7.1 107 5.6 6.9 112
10 nM 8.7 6.8 87 9.5 1.9 95
50 nM 47 1.3 94 47 1.9 94
100 nM 88 2.1 88 90 1.5 90
250 nM 221 3.3 88 225 3.1 90
1000 nM
975
0.9
97
978
1.1
98