Kinetic parameters for 2-hydroxylation of E2 by pooled HLM, CYP3A4, and CYP1A2
Values are expressed as mean ± S.E.M. (n = 3).
Km | Vmax | Vmax/Km | |
|---|---|---|---|
| μM | nmol/min/mg protein | μl/min/mg protein | |
| HLM | 42.8 ± 9.40 | 0.32 ± 0.02 | 7.5 |
| nmol/min/nmol P450 | μl/min/nmol P450 | ||
| CYP3A4 | 23.7 ± 3.00 | 6.9 ± 0.35 | 291 |
| CYP1A2 | 27.5 ± 2.16 | 17.4 ± 0.54 | 633 |