TABLE 1

Site-directed mutagenesis primers sequences for UGT1A8 variants

Variant positions are in bold.

UGT1A8 Variants	Primer Sequence^a
S⁴³L	(F) 5′-GGTTCACCATGCAGTTGGTGGTGGAGAAACT
	(R) 5′-AGTTTCTCCACCACCAACTGCATGGTGAACC
H⁵³N	(F) 5′-CTTATCCTCAGGGGGAATGAGGTGGTTGTAG
	(R) 5′-CTACAACCACCTCATTCCCCCTGAGGATAAG
S¹²⁶G	(F) 5′-TTCGCATTGCAGGGGTTTGTTTAATGACCG
	(R) 5′-CGGTCATTAAACAAACCCCTGCAATGCGAA
A¹⁴⁴V	(F) 5′-GGAGAGTTCTTTTGATGTGGTGTTTCTTGATCC
	(R) 5′-GGATCAAGAAACACCACATCAAAAGAACTCTCC
A²³¹T	(F) 5′-GCCCTAGAAATAACCTCTGAAATTCTCC
	(R) 5′-GGAGAATTTCAGAGGTTATTTCTAGGGC
T²⁴⁰A	(F) 5′-CTCCAAACACCTGTCGCAGCATATGATCTC
	(R) 5′-GAGATCATATGCTGCGACAGGTGTTTGGAG