TABLE 1

Phase I and phase II activities in microsomes prepared from steatotic and nonsteatotic human liver grafts

Activity
CYP
Nonsteatosisa
Steatosis
Percentageb
Nonsteatosisc
Steatosis
Percentageb
Phase I
    CPR 72 ± 20 66 ± 23 92
    ECOD Several 417 ± 240 300 ± 88 72 6.1 ± 3.8 4.9 ± 0.9 80
    MROD 1A2 25 ± 16 11 ± 5* 44 0.35 ± 0.19 0.16 ± 0.08* 46
    CH 2A6 296 ± 164 370 ± 130 125 3.7 ± 2.2 5.1 ± 2.1 139
    D4OH 2C9 1.8 ± 1.0 1.4 ± 0.4 78 28 ± 19 23 ± 8 82
    C6OH 2E1 6.9 ± 5.8 5.7 ± 3.0 83 110 ± 98 96 ± 34 87
    6βOHT 3A4 727 ± 403 360 ± 255* 50 10.8 ± 5.7 6.0 ± 4.0* 55
Phase II
    GT
6.1 ± 2.2
8.7 ± 4.1
143

  • ↵ a Activities were measured in liver microsomes using selective substrates and expressed as picomoles of corresponding metabolite formed per minute and per milligram of protein, except in the case of CPR, D4OH, C6OH, and GT, which were expressed in nanomoles per minute and per milligram of protein. Results are mean ± S.D. of different microsomal preparations from nonsteatotic (n = 10) or steatotic (n = 9) livers.

  • ↵ b Percentage with respect to the corresponding activity value in nonsteatosis group.

  • ↵ c For each microsomal preparation, phase I activities were normalized respect to CPR activity values. Results are mean ± S.D. of different microsomal preparations from nonsteatotic (n = 10) or steatotic (n = 9) livers.

  • ↵* p < 0.05 with respect to nonsteatosis group.