Urinary and fecal excretion of benzylic mercapturic acids (MPMA and DMPMA) by rats treated with 1-hydroxymethylpyrene and 1-hydroxymethyl-8-methylpyrene, respectively
Each test compound (83.3 μmol/kg body weight) was intraperitoneally administrated to five male Wistar (Han) rats. One animal treated with 1-hydroxymethylpyrene had to be discarded due to puncturing of the cecum. Urine and feces were collected in metabolic cages for 24 h after the treatment. They were analyzed for levels of mercapturic acids using UPLC-MS/MS. Data are expressed in ppm of the dose and percentage of total mercapturic acid excreted (in urine plus feces) for each animal. The percentage of urinary and fecal excretion was significantly different between the compounds (p < 0.01, Mann-Whitney U test).
Mercapturic Acid | Animal | Dose | Route | ||||
|---|---|---|---|---|---|---|---|
| Urine | Feces | Urine | Feces | ||||
| ppm | % of total | ||||||
| MPMA | 1 | 43 | 40 | 52 | 48 | ||
| 2 | 314 | 20 | 94 | 6 | |||
| 3 | 151 | 57 | 73 | 27 | |||
| 4 | 57 | 27 | 68 | 33 | |||
| Mean ± S.D. | 141 ± 125 | 36 ± 16 | 72 ± 17 | 28 ± 17 | |||
| DMPMA | 1 | 1.3 | 3.0 | 30 | 70 | ||
| 2 | 0.7 | 19.4 | 4 | 96 | |||
| 3 | 1.8 | 13.2 | 12 | 89 | |||
| 4 | 1.7 | 14.9 | 10 | 90 | |||
| 5 | 1.4 | 59.9 | 2 | 98 | |||
|
| Mean ± S.D. | 1.4 ± 0.4 | 22 ± 22 | 12 ± 11 | 88 ± 11 | ||