Summary of bendamustine metabolites detected in rat bile using LC-MSn
Peak Identification | Retention Time | [M + H]+ (12C) | Rat Bile | ||||
|---|---|---|---|---|---|---|---|
| 0-30 min | 30-60 min | 60-120 min | |||||
| min | |||||||
| P | 36.7 | 358 | √ | √ | N.R. | ||
| M5 | 18.4 | 276 | √ | √ | N.R. | ||
| M12 | 26.7 | 501 | N.D. | √ | N.D. | ||
| M15 | 28.3 | 612 | √ | √ | √ | ||
| M16 | 30.8 | 340 | √ | N.D. | N.D. | ||
| M17 | 30.3 | 354 | N.D. | √ | √ | ||
| M18 | 31.6 | 501 | √ | √ | √ | ||
| M19 | 32.9 | 485 | √ | √ | √ | ||
| M20 | 35.0 | 374 | √ | √ | N.D. | ||
| M21 | 36.4 | 344 | N.D. | √ | N.D. | ||
P, parent bendamustine; M followed by number, metabolite; √, detected; N.D., not detected; N.R., detected by LC-MS, but not by the radiochemical detector.