Summary of bendamustine metabolites detected in rat urine using LC-MSn
Metabolites M7 and M9 were observed at low levels in some samples, but were not characterized.
Peak Identification | Retention Time (min) | [M + H]+ (12C) | Rat Urine | ||||
|---|---|---|---|---|---|---|---|
| 0-4 h | 4-8 h | 8-24 h | |||||
| min | |||||||
| P | 35.6 | 358 | √ | √ | √ | ||
| M1 | 12.4 | 320 | √ | √ | N.D. | ||
| M2 | 13.6 | 292 | √ | N.D. | √ | ||
| M3 | 15.6 | 278 | N.D. | N.D. | √ | ||
| M4 | 15.8 | 292 | √ | N.D. | √ | ||
| M5 | 17.6 | 276 | √ | √ | √ | ||
| M6 | 18.9 | 322 | √ | N.D. | √ | ||
| M8 | 23.3 | 547 | √ | N.D. | N.D. | ||
| M10 | 24.9 | 467 | √ | N.D. | N.D. | ||
| M11 | 25.5 | 641 | √ | N.D. | N.D. | ||
| M12 | 26.5 | 501 | √ | N.D. | N.D. | ||
| M13 | 26.9 | 641 | √ | N.D. | N.D. | ||
| M14 | 27.9 | 595 | √ | N.D. | N.D. | ||
| M15 | 27.9 | 612 | √ | N.D. | N.D. | ||
| M16 | 28.9 | 340 | √ | N.D. | N.D. | ||
| M18 | 30.8 | 501 | √ | N.D. | N.D. | ||
| M19 | 31.7 | 485 | √ | √ | √ | ||
P, parent bendamustine; M followed by number, metabolite; √, detected; N.D., not detected.