TABLE 3

Probe substrates and substrate concentrations used to characterize the rates and routes of metabolism in human freshly isolated lung parenchymal cells and human pooled cryopreserved hepatocytes

Rate concentrations were carried out at 1 μm and 2 μm in hepatocytes and LPCs, respectively.

Substrate	Rate Incubations in Hepatocytes	Rate Incubations in LPCs	Route Incubations in Hepatocytes and LPCs	K_m of Reaction^a
	μM			μM
7-EC	1	2	25	3.9 μM (1)
ER	1	2	20	0.18-0.21 μM (2)
Coumarin	1	2	25	0.3-2.3 μM (2)
Bupropion	1	2	50	67-168 μM (2)
Diclofenac	1	2	50	3.4-52 μM (2)
S-Mephenytoin	1	2	100	13-35 μM (2)
Bufuralol	1	2	20	9-15 μM (2)
Astemizole	1	2	50	1.8 μM (3)
Midazolam	1	2	25	1-14 μM (2)
Testosterone	1	2	100	52-94 μM (2)
7-HC	1	2	25	UGT 79 μM (4)
				SULT ~3 μM (4)
1-Naphthol	1	2	25	40.2 μM (5)
4-methyl umbelliferone	1	2	25	920 μM (6)
Acetaminophen	1	2	25	UGT 2.2 mM (7)
				SULT <1 mM (8)
BDP	1	2	50
Salbutamol	1	2	25	142 μM (9)
Budesonide	1	2	25	126.9 μM (10)
Formoterol	1	2	25	827.6 μM (11)

↵ a (1) Bayliss et al., 1994; (2) Huang and Stifano, 2006; (3) Matsumoto et al., 2002; (4) Wang et al., 2006; (5) Cho et al., 2006; (6) Green et al., 1998; (7) Mutlib et al., 2006; (8) Kane et al., 1995; (9) Pacifici et al., 1996; (10) Sudsakorn et al., 2005; (11) Zhang et al., 2000.