List of oligonucleotide probes generated for use in bDNA signal amplification assay
Targeta | Functionb | Sequence | Target | Function | Sequence |
|---|---|---|---|---|---|
| AhR (NM_013464) | CAR (NM_009803) | ||||
| 2766-2784 | CE | cttccgccaggtgatggagTTTTTctcttggaaagaaagt | 42-64 | CE | ttccttgagatctcaggaactgcTTTTctcttggaaagaaagt |
| 2841-2865 | CE | ggtatcctgttcctgaatgaatttcTTTTTctcttggaaagaaagt | 84-102 | CE | gatgcctcctctccccaggTTTTctcttggaaagaaagt |
| 3004-3024 | CE | tgtgcactctgaggtgcctgaTTTTTctcttggaaagaaagt | 190-209 | CE | cagttcctcggcccatattcTTTTctcttggaaagaaagt |
| 3072-3094 | CE | ccaaatacctccctgtttttcctTTTTTctcttggaaagaaagt | 271-291 | CE | tgttcgtctgaagaagcccttTTTTTctcttggaaagaaagt |
| 2705-2726 | LE | gtcggacgaataggtttcattgTTTTTaggcataggacccgtgtct | 22-41 | LE | caggaattagggactgcggtTTTTaggcataggacccgtgtct |
| 2727-2745 | LE | cgtggccaatgctgctcaaTTTTTaggcataggacccgtgtct | 65-83 | LE | cctcctcgctgaccctgctTTTTTaggcataggacccgtgtct |
| 2821-2840 | LE | atcctggcatgggagctacaTTTTTaggcataggacccgtgtct | 124-143 | LE | tcctgtttcccttaggcagcTTTTTaggcataggacccgtgtct |
| 2866-2888 | LE | tgatgtccaaccctcacagttctTTTTTaggcataggacccgtgtct | 169-189 | LE | ttcttcactggccatggtttcTTTTTaggcataggacccgtgtct |
| 2943-2963 | LE | gggagcatctcaggtacgggtTTTTTaggcataggacccgtgtct | 210-229 | LE | cccggtctccacacaccacaTTTTTaggcataggacccgtgtct |
| 2964-2984 | LE | tccgagctctcccatcgtataTTTTTaggcataggacccgtgtct | 251-270 | LE | gcagccctcacaagtcagggTTTTTaggcataggacccgtgtct |
| 2985-3003 | LE | actcctcccatgtgctccgTTTTTaggcataggacccgtgtct | 292-312 | LE | gggaccaatggttttgctgacTTTTTaggcataggacccgtgtct |
| 3025-3049 | LE | cccgagaatttttcacagtaaacacTTTTTaggcataggacccgtgtct | 313-333 | LE | ccttccagcaaacggacagatTTTTTaggcataggacccgtgtct |
| 2746-2765 | BL | atggccagtggtctgagcag | 335-353 | LE | tgggccttgctgacctcacTTTTTaggcataggacccgtgtct |
| 2785-2800 | BL | tcgggaagaggccggg | 103-123 | BL | aggccacaagattgggtgtag |
| 2801-2820 | BL | ggaatccaccgggtgtgata | 144-168 | BL | tagtgttagcatagctgtcatggtc |
| 2889-2914 | BL | cgaaatctgttttggagaaagtgtac | 230-250 | BL | cgtggaaatgatagcctgtgg |
| 2915-2942 | BL | tttaaactccttctctaaacacaagaat | |||
| 3050-3071 | BL | gctgaagttactgagcagggaa | |||
| PXR (AF031814) | PPARα (NM_011144) | ||||
| 495-510 | CE | ggcccgcctctgctccTTTTTctcttggaaagaaagt | 1245-1265 | CE | gaagaatcggacctctgcctcTTTTTctcttggaaagaaagt |
| 571-589 | CE | gcgcctgctgttcttccgtTTTTTctcttggaaagaaagt | 1453-1473 | CE | actcgcgtgtgataaagccatTTTTTctcttggaaagaaagt |
| 762-784 | CE | gctgcagagagatcttcattggaTTTTTctcttggaaagaaagt | 1474-1495 | CE | ggcttcctcaggttcttaaggaTTTTTctcttggaaagaaagt |
| 843-864 | CE | tggcagaagagggatgatctctTTTTTctcttggaaagaaagt | 1611-1632 | CE | agcctatgtttagaaggccaggTTTTTctcttggaaagaaagt |
| 435-452 | LE | tccaggcacttgcgcaaaTTTTTaggcataggacccgtgtct | 1226-1244 | LE | tttgtcttcgacgccgttgTTTTTaggcataggacccgtgtct |
| 477-494 | LE | acagcggcatcggacatgTTTTTaggcataggacccgtgtct | 1286-1303 | LE | tccgtgacggtctccacgTTTTTaggcataggacccgtgtct |
| 590-610 | LE | cgtccatcagctcctggatcaTTTTTaggcataggacccgtgtct | 1368-1392 | LE | cttcatacacaccgtactttagcaaTTTTTaggcataggacccgtgtct |
| 611-633 | LE | tgtgtcaaaggtttgcatctgagTTTTTaggcataggacccgtgtct | 1414-1434 | LE | tcccgtctttgttcatcaaggTTTTTaggcataggacccgtgtct |
| 718-737 | LE | catgtggcagggtcttccaaTTTTTaggcataggacccgtgtct | 1435-1452 | LE | tgccgtacgcgatcagcaTTTTTaggcataggacccgtgtct |
| 738-761 | LE | accctgtctttcatgatttgactcTTTTTaggcataggacccgtgtct | 1496-1518 | LE | tgggttccatgatgtcacagaacTTTTTaggcataggacccgtgtct |
| 785-801 | LE | gccgtcttctccgcgcaTTTTTaggcataggacccgtgtct | 1544-1566 | LE | cactgtcatccagttctaaggcaTTTTTaggcataggacccgtgtct |
| 865-883 | LE | ttgacacatcggccaggtgTTTTTaggcataggacccgtgtct | 1633-1654 | LE | ccctcctgcaacttctcaatgtTTTTTaggcataggacccgtgtct |
| 453-476 | BL | atcatctctttcttcatgccactc | 1655-1676 | LE | gagcttaagcacgtgcacaatcTTTTTaggcataggacccgtgtct |
| 511-533 | BL | tccctcttcttcctcttgatcaa | 1677-1696 | LE | ggatggttgctctgcaggtgTTTTTaggcataggacccgtgtct |
| 534-553 | BL | gcggtggagcctcaatcttt | 1266-1285 | BL | gacatgcactggcagcagtg |
| 554-570 | BL | cagcccctgccctccag | 1304-1324 | BL | gccttggcaaattctgtgagc |
| 634-657 | BL | gaaatccttgaagtgggagaaagt | 1325-1345 | BL | aagtttgcaaagcctgggata |
| 658-675 | BL | gaacactgcaggcagccg | 1346-1367 | BL | ggtgacttggtcgttcaagtcc |
| 676-696 | BL | tggaagctcacagccactgtg | 1393-1413 | BL | aggacagcatcgtgaagatgg |
| 697-717 | BL | cagtgaggcctgcagaaactc | 1519-1543 | BL | ttgaacttcatagcgaagtcaaact |
| 802-823 | BL | ggggttggtagttccagatgct | 1567-1587 | BL | cagccacaaacagggaaatgt |
| 824-842 | BL | ttcccgtcgctcttggaag | 1588-1610 | BL | ccgatctccacagcaaattatag |
| Nrf2 (NM_010902) | |||||
| 50-67 | CE | tgctcgctggagtggcagTTTTTctcttggaaagaaagt | |||
| 110-127 | CE | gcagcagaggtgaggcggTTTTTctcttggaaagaaagt | |||
| 322-345 | CE | ttccttttcgagttttttctgtttTTTTTctcttggaaagaaagt | |||
| 32-49 | LE | gccagccttagtccgcccTTTTTaggcataggacccgtgtct | |||
| 68-88 | LE | gccgggaactaggagatagccTTTTTaggcataggacccgtgtct | |||
| 89-109 | LE | cggcaatggctagtccgagtaTTTTTaggcataggacccgtgtct | |||
| 128-145 | LE | ccgacggcgaggctacttTTTTTaggcataggacccgtgtct | |||
| 146-164 | LE | gacgctgtggtagggctccTTTTTaggcataggacccgtgtct | |||
| 165-182 | LE | tccatcatgctgagggcgTTTTTaggcataggacccgtgtct | |||
| 183-200 | LE | ggcggtggcaactccaagTTTTTaggcataggacccgtgtct | |||
| 201-222 | LE | gtcctgctgggactgtagtcctTTTTTaggcataggacccgtgtct | |||
| 223-246 | LE | ccaaaggatgtcaatcaaatccatTTTTTaggcataggacccgtgtct | |||
| 298-321 | LE | ttccaactcatagtccttctgtcgTTTTTaggcataggacccgtgtct | |||
| 247-271 | BL | ttactccaagatctatgtcttgcct | |||
| 272-297 | BL | ctgactaaagtcaaacacttctcgac |
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CE, capture extender; LE, label extender; BL, blocker.
↵ a Target sequence refers to the region of the mRNA transcript to which a given probe is complementary, GenBank accession numbers for each transcript are given in parenthesis next to the gene name.
↵ b Function refers to the type of bDNA oligonucleotide probe represented by each sequence.