TABLE 4

Kinetics of bisphenol A glucuronidation by cryopreserved female human HCs with and without the addition of exogenous UDPGA

The 10-min incubations contained 125,000 HCs/0.5 ml, various concentrations of [¹⁴C]BPA (1.3-52 μM), and either 0, 50, or 1000 μM UDPGA. All data were converted to the rate of BPA-glucuronide formed per minute per 10⁶ HCs. CL_int and CL_met determinations were carried out as described under Materials and Methods.

Human Specimens	V_max	K_m	V_max/K_m	Scaled CL_int	Scaled CL_met
	pmol/min/10⁶ hepatocytes	μM	μl/min/10⁶ hepatocytes	ml/min/kg
HFH 1	510	9	54	143	6
HFH 2	234	8	28	73	4
HFH 3	840	7	127	336	10
HFH 4	396	11	35	92	4
HFH 5	560	7	85	224	8
HFH 6	561	10	54	143	6
HFH 7	260	5	55	146	6
No UDPGA added^a	480 ± 208	8 ± 2	63 ± 34	165 ± 89	6 ± 2
50 μM UDPGA^b	1004 ± 324	15 ± 8	67 ± 31	179 ± 84	7 ± 3
1000 μM UDPGA^b	3162 ± 1256	18 ± 5	165 ± 102	483 ± 285	12 ± 4

↵ a Mean ± S.D. for n = 7 (no UDPGA added).
↵ b Mean ± S.D. for n = 6 (50 or 1000 μM UDPGA).