Oligonucleotide sequences used for electrophoretic mobility shift assays
The hexamer half-sites are capitalized. The nucleotides that are different from the consensus hexamer are shown with bold letters. Mutated sites are underlined.
Probe | Sequence |
|---|---|
| cEREa | 5′-gtccaaagtcAGGTCAcagTGACCTgatcaaagtt-3′ |
| 2A6/ERE | 5′-cctccctggccAGGTCAtggCAACCTgctgatcac-3′ |
| 2A6/ERE mut | 5′-cctccctggccAGTTCAtggCAAACTgctgatcac-3′ |
↵ a The consensus ERE in Xenopus vitellogenin A2 promoter (Tsuchiya et al., 2004).