Nucleotide sequences of PCR primers
Gene | Primer Sequence (5′-3′) | Denaturation | Annealing | Elongation |
|---|---|---|---|---|
| Mrp2 | ||||
| Forward | accttccacgtagtgatcct | 95°C (1 s) | 57°C (30 s) | 72°C (30 s) |
| Reverse | acctgctaagatggacggtc | |||
| Mrp3 | ||||
| Forward | gtgctgaagaatttgactctg | 95°C (1 s) | 60°C (10 s) | 72°C (40 s) |
| Reverse | gaccaggacccggttgtagtc | |||
| Bsep | ||||
| Forward | cactgggtacatgtggtgtctcat | 95°C (10 s) | 60°C (30 s) | 72°C (30 s) |
| Reverse | atggccaatattcatagctgctaat | |||
| 18S | ||||
| Forward | gtaacccgttgaaccccatt | 95°C (1 s) | 55°C (30 s) | 72°C (30 s) |
| Reverse | ccatccaatcggtagtagcg |
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