TABLE 2

Effect of organic solvents on the kinetics of CYP2C8-mediated paclitaxel hydroxylation in the presence of HLM

Kinetic parameters are expressed as % Control (mean ± S.D.)

	Solvent (%, v/v)
	Methanol	Ethanol	Acetonitrile	DMSO
K _m
0 (Control)	100.0 ± 1.4	100.0 ± 3.2	100.0 ± 2.2	100.0 ± 4.0
0.2	67.8 ± 1.7^a	165.3 ± 12.1^b	101.9 ± 0.5	123.7 ± 2.4^b
0.5	65.1 ± 2.4^a	303.4 ± 14.5^a	101.5 ± 0.1	128.8 ± 8.0^b
1	N.E.	N.E.	92.9 ± 16.5	N.E.
2	132.0 ± 7.6^b	507.4 ± 26.5^a	132.3 ± 2.2^a	139.0 ± 1.6^a
5	166.6 ± 6.4^a	893.4 ± 53.4^a	183.8 ± 12.0^b	202.8 ± 7.2^a
V _max
0 (Control)	100.0 ± 3.2	100.0 ± 3.4	100.0 ± 2.1	100.0 ± 2.0
0.2	96.1 ± 2.9	105.0 ± 5.4	105.3 ± 2.5	102.4 ± 3.3
0.5	97.4 ± 2.6	112.6 ± 6.5	109.2 ± 2.2	102.2 ± 1.2
1	N.E.	N.E.	96.6 ± 6.5	N.E.
2	114.6 ± 6.3	131.4 ± 9.4	101.9 ± 2.7	102.9 ± 3.1
5	124.5 ± 3.8^b	96.9 ± 0.8	76.3 ± 3.3^b	98.2 ± 3.3
CL_int
0 (Control)	100.0 ± 4.6	100.0 ± 0.2	100.0 ± 4.3	100.0 ± 6.0
0.2	141.7 ± 7.7^b	63.5 ± 1.4^c	103.2 ± 1.9	82.6 ± 1.0
0.5	149.8 ± 9.5^b	37.1 ± 0.4^c	107.5 ± 2.2	79.4 ± 5.8
1	N.E.	N.E.	103.4 ± 11.5	N.E.
2	86.8 ± 0.2^b	25.9 ± 0.5^c	77.0 ± 3.3^b	74.0 ± 3.1^b
5	74.7 ± 0.5^b	10.9 ± 0.6^c	41.5 ± 0.9^a	48.4 ± 0.1^a

N.E. = Not evaluated.
↵ a Significantly different from control: P < 0.01, Student's t test.
↵ b Significantly different from control: P < 0.05, Student's t test.
↵ c Significantly different from control: P < 0.001, Student's t test.