Correlation analysis between the extent of CYP2A immunoinhibition and the degree of NNK biotransformation, levels of CYP2A mRNA, and 7-hydroxycoumarin formation
Percentage Change in Total α-Hydroxylationa | Percentage Change in Total N-Oxidation | Percentage Change in NNAL Formation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Total α-hydroxylation (n = 29) | r = 0.234b | P = 0.222b | ||||||||
| High bioactivation group | r = 0.285 | P = 0.268 | ||||||||
| Low bioactivation group | r = 0.313 | P = 0.322 | ||||||||
| Total N-oxidation (n = 29) | r = 0.177 | P = 0.360 | ||||||||
| High detoxification group | r = 0.218 | P = 0.573 | ||||||||
| Low detoxification group | r = 0.156 | P = 0.512 | ||||||||
| NNAL formation (n = 29) | r = −0.0362 | P = 0.852 | ||||||||
| CYP2A13 mRNA (n = 28) | r = 0.0861 | P = 0.633 | r = 0.0586 | P = 0.767 | r = 0.112 | P = 0.569 | ||||
| CYP2A6 mRNA (n = 24) | r = −0.338 | P = 0.107 | r = 0.0815 | P = 0.705 | r = −0.0234 | P = 0.960 | ||||
| 7-Hydroxycoumarin formation (n = 27) |
| r = 0.0200 | P = 0.920 | r = 0.125 | P = 0.533 | r = 0.00856 | P = 0.966 | |||
↵ a CYP2A6/13 immunoinhibition was determined by the percentage change in NNK metabolism in the presence of a CYP2A6/13 inhibitory antibody. Immunoinhibition experiments were performed independently of other experiments that assessed NNK metabolism, mRNA expression, and coumarin 7-hydroxylation.
↵ b Correlation coefficients (r) and P values were determined using Pearson's correlation analysis.