TABLE 2
Comparisons of the kinetic parameters of midazolam 1′-hydroxylation by the CYP3A4.1 and CYP3A4.18 proteins
The values are estimated from nonlinear least-squares regression analysis using WinNonlin software (Pharsight). The reactions include equal amounts of P450 (5 pmol) from the CO spectrum analysis, not P450 + P420. Detailed procedures are described under Materials and Methods.