Isoform Monitored | Marker Substrate | Substrate Concentration | Incubation Time | Protein Concentration | Metabolite Formed |
---|---|---|---|---|---|
μM | min | mg/ml | |||
CYP1A2 | Phenacetin | 50 | 30 | 0.1 | Acetaminophen |
CYP2A6 | Coumarin | 1 | 5 | 0.025 | 7-Hydroxycoumarin |
CYP2B6 | Bupropion | 125 | 20 | 0.25 | Hydroxybupropion |
CYP2C9 | Tolbutamide | 140 | 20 | 0.1 | Hydroxytolbutamide |
CYP2C19 | (S)-Mephenytoin | 50 | 30 | 0.1 | 4′-Hydroxymephenytoin |
CYP2D6 | Dextromethorphan | 7.5 | 30 | 0.1 | Dextrorphan |
CYP3A4 | Midazolam | 5 | 4 | 0.025 | 1′-Hydroxymidazolam |
CYP3A4 | Testosterone | 50 | 7 | 0.05 | 6β-Hydroxytestosterone |