Comparison of kinetic parameters for recombinant zmSHMT(DelSig) with zcSHMT and rmSHMT
Apparent Km and Vmax values were determined by fitting the data to the Michaelis-Menten equation using Sigma Plot.
Species | αKm for serine (mM) | αKd for H4PteGlu (μM) | αKd for N5-CHO-H4PteGlu (μM) | Turnover Number (min−1) | References |
|---|---|---|---|---|---|
| Zebrafish ma | 0.43 | 8 | 2.8 | 537 | Present report |
| Zebrafish cb | 0.22 | 18 | 2.6 | 351 | Chang et al. (Chang et al., 2006) |
| Rabbit mc | 0.60 | 25 | 10 | 500 | Schirch et al. (Schirch and Peterson, 1980) |
a zebrafish mSHMT(DelSig); b cytosolic SHMT; c mitochondrial SHMT