Kinetic parameters for the transcellular transport of olmesartan in OATP1B1/MRP2 double transfectant
Data shown in Fig. 3 were used to determine these parameters calculated by nonlinear regression analysis as described under Materials and Methods. Each parameter represents the mean ± computer-calculated S.D.
Km | Vmax | Pdif | Vmax/Km | |
|---|---|---|---|---|
| μM | pmol/min/mg protein | μl/min/mg protein | μl/min/mg protein | |
| Transcellular transport | 39.3 ± 9.1 | 178 ± 43 | 1.20 ± 0.18 | 4.54 ± 0.24 |
|
PS
apical
| 45.5 ± 16.9 | 158 ± 68 | 2.08 ± 0.32 | 3.48 ± 0.37 |
PSapical, the efflux clearance on the apical membrane.